Development of the mammalian axial skeleton requires signaling through the G alpha(i) subfamily of heterotrimeric G proteins

作者：Plummer Nicholas W^*; Spicher Karsten; Malphurs Jason; Akiyama Haruhiko; Abramowitz Joel; Nuernberg Bernd; Birnbaumer Lutz

来源：Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(52): 21366-21371.

DOI：10.1073/pnas.1219810110

• mouse
• thoracic
• sternum
• lateral plate mesoderm

129/SvEv mice with a loss-of-function mutation in the heterotrimeric G protein alpha-subunit gene Gnai3 have fusions of ribs and lumbar vertebrae, indicating a requirement for Gai (the %26quot;inhibitory%26quot; class of alpha-subunits) in somite derivatives. Mice with mutations of Gnai1 or Gnai2 have neither defect, but loss of both Gnai3 and one of the other two genes increases the number and severity of rib fusions without affecting the lumbar fusions. No myotome defects are observed in Gnai3/Gnai1 double-mutant embryos, and crosses with a conditional allele of Gnai2 indicate that G alpha(i) is specifically required in cartilage precursors. Penetrance and expressivity of the rib fusion phenotype is altered in mice with a mixed C57BL/6 x 129/SvEv genetic background. These phenotypes reveal a previously unknown role for G protein-coupled signaling pathways in development of the axial skeleton.

• 出版日期2012-12-26