A squaraine-based red emission off-on chemosensor for biothiols and its application in living cells imaging

作者:Liu, Xiao-Dong; Sun, Ru; Ge, Jian-Feng*; Xu, Yu-Jie; Xu, Ying; Lu, Jian-Mei
来源:Organic and Biomolecular Chemistry, 2013, 11(25): 4258-4264.
DOI:10.1039/c3ob40502h

摘要

A new probe based on the squaraine skeleton with a 2,4-dinitrobenzenesulfonyl unit is reported as a fluorescent probe for biothiol. It shows turn-on properties with red emission towards biothiols in acetonitrile-PBS buffer (1 : 9, v/v) solution, and its properties of rapid response, high selectivity and high sensitivity make it a potential probe with real applications. Moreover, the in vitro assays show that the probe can be used in fluorescence imaging for the detection of intracellular biothiol levels.