A universal label-free fluorescent aptasensor based on Ru complex and quantum dots for adenosine, dopamine and 17 beta-estradiol detection

作者：Huang, Hailiang; Shi, Shuo^*; Gao, Xing; Gao, Ruru; Zhu, Ying; Wu, Xuewen; Zang, Ruimin; Yao, Tianming^*

来源：Biosensors and Bioelectronics, 2016, 79: 198-204.

DOI：10.1016/j.bios.2015.12.024

• Ru complex
• Quantum dots
• Adenosine
• Dopamine
• 17 beta-estradiol
• Fluorescence

Based on specific aptamer binding properties, a strategy for adenosine, dopamine and 17 beta-estradiol detection was realised by employing Ru complex and quantum dots (QDs) as fluorescence probes. Ru complex, which could quench the fluorescence of QDs, preferred to bind with aptamer DNA and resulted in the fluorescence rise of QDs. When the aptamer DNA was incubated with the target first, it could not bind with Ru complex and the fluorescence of QDs was quenched. Under the optimal condition, the fluorescence intensity was linearly proportional to the concentration of adenosine, dopamine and 17 beta-Pestradiol with a limit of detection (LOD) of 101 nM, 19 nM and 37 nM, respectively. The experiments in fetal bovine serum were also carried out with good results. This universal method was rapid, label-free, low-cost, easy-operating and highly repeatable for the detection of adenosine, dopamine and 17 beta-estradiol. Qualitative detection by naked eyes was also available without complex instruments. It could also be extended to detect various analytes, such as metal ions, proteins and small molecules by using appropriate aptamers.

• 出版日期2016-5-15
• 单位同济大学