Neutrophil infiltration mediated by CXCL5 accumulation in the laryngeal squamous cell carcinoma microenvironment: A mechanism by which tumour cells escape immune surveillance

作者：Zhang, Duo; Zhou, Jian; Tang, Di; Zhou, Lin; Chou, Liang; Chou, Kuang-yen; Tao, Lei^*; Lu, Li-ming^*

来源：Clinical Immunology, 2017, 175: 34-40.

DOI：10.1016/j.clim.2016.11.009

• CXCL5
• Neutrophil
• LSCC
• Tumour microenvironment
• TANs
• PBNs

The CXCL5 chemokine is important for neutrophil accumulation in tumour tissues. In this report, we attempted to clarify whether and how infiltrating tumour-associated neutrophils (TANS) in laryngeal squamous cell carcinoma (LSCC) affect the proliferation and activation of T cells. We examined chemokine expression by real-time PCR (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) and performed an immunohistochemical analysis of LSCC microarrays. The relationship between CXCL5 and CD66b (a neutrophil marker) was investigated by immunofluorescence staining. We found that CXCL5 was upregulated in LSCC tissues, whereas CXCL5 levels were decreased in LSCC patient serum. Furthermore, high levels of CXCL5 were significantly correlated with intratumoural neutrophil infiltration. Compared with peripheral blood neutrophils (PBNs), TANs significantly inhibited T cell proliferation and decreased IFN-gamma and TNF-alpha secretion. These data suggest that excessive neutrophil infiltration is associated with advanced clinical stages of LSCC (T3 or T4, III or IV, and N1 or N2).

• 出版日期2017-2
• 单位上海交通大学; 复旦大学