Use of a combination of in vitro models to investigate the impact of chlorpyrifos and inulin on the intestinal microbiota and the permeability of the intestinal mucosa

作者：Requile Marina; Alvarez Duban O Gonzalez; Delanaud Stephane; Rhazi Larbi; Bach Veronique; Depeint Flore; Khorsi Cauet Hafida^*

来源：Environmental Science and Pollution Research, 2018, 25(23): 22529-22540.

DOI：10.1007/s11356-018-2332-4

• Caco-2/TC7
• Chlorpyrifos
• Inulin
• Dysbiosis
• Microbiota
• SHIME (R)

Dietary exposure to the organophosphorothionate pesticide chlorpyrifos (CPF) has been linked to dysbiosis of the gut microbiota. We therefore sought to investigate whether (i) CPF's impact extends to the intestinal barrier and (ii) the prebiotic inulin could prevent such an effect. In vitro models mimicking the intestinal environment (the SHIMEA (R)) and the intestinal mucosa (Caco-2/TC7 cells) were exposed to CPF. After the SHIMEA (R) had been exposed to CPF and/or inulin, we assessed the system's bacterial and metabolic profiles. Extracts from the SHIMEA (R)'s colon reactors were then transferred to Caco-2/TC7 cultures, and epithelial barrier integrity and function were assessed. We found that inulin co-treatment partially reversed CPF-induced dysbiosis and increased short-chain fatty acid production in the SHIMEA (R). Furthermore, co-treatment impacted tight junction gene expression and inhibited pro-inflammatory signaling in the Caco-2/TC7 intestinal cell line. Whereas, an isolated in vitro assessment of CPF and inulin effects provides useful information on the mechanism of dysbiosis, combining two in vitro models increases the in vivo relevance.

• 出版日期2018-8