Analysis of Foxp3, CD25, and CD127 Expressed on Regulatory T Cells in Thai Subjects

作者：Thongpan Masatha; Kitiyakara Chagriya; Louischaroen Yada; Keesukphan Prasit; Chaiyaroj Sansanee C^*

来源：Asian Pacific Journal of Allergy and Immunology, 2009, 27(2-3): 137-145.

Foxp3(+) natural regulatory T cells (nTreg) play a distinct role in maintaining self tolerance at the periphery. CD25(hi) and CD127(lo) were proposed for the identification and purification of nTreg but they have not been confirmed in non-Caucasian populations. This study examined the sensitivity and purity of Foxp3 nTreg identified by CD25 and CD127 in the peripheral blood of Thai subjects (13 males, 15 females with age range of 20-42 years old). The proportions of nTreg/CD4(+) as identified by the different markers were as follows: Foxp3(+), 18.3 +/- 6.4%; CD25(hi) 6.4 +/- 3.2%; and CD127(lo), 54.3 +/- 14.2%. Sensitivity tests showed the following results: CD25(hi), 23.1%; CD127(lo), 40.6%; CD25(hi)CD127(lo), 7.4%. Purity tests concluded as follows: CD25(hi), 63.6%; CD25(int), 24.9%; CD25(lo), 8.7%, CD127(lo), 26.5%; CD127(hi), 14.9%, and CD25(hi)CD127(lo), 52.0%. In conclusion, the proportions of nTreg in Thai subjects are h similar to Caucasian populations. CD25(hi) is superior to CD127(lo) for separating Foxp3(+) nTreg. Combining CD25(hi) and CD127(lo) does not improve the nTreg purity.

• 出版日期2009-9