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摘要
For analysis of a bunch of samples, a rapid, selective, sensitive and precision method in EN 12868:1999 was developed to determine the release of N-nitrosamines and N-nitrosatable substances from elastomer or rubber teats and soothers in both of sample preparation and instrumental analysis. To imitate the migration of N-nitrosamines and N-nitrosatable substances from rubber balloons and teats to human body, rubber products studied in this research were soaked in artificial saliva. The target analytes released to artificial saliva were extracted by Sep-Pak AC2 connected to Sep-Pak Dry cartridges, and isolated by DB 624 GC column, and determined by positive chemical ionization mass spectrometry in both full scan and SIM mode. Linear concentration-response relationships were obtained in a calibration range from 10 to 1000 mu g/L with high correlation(R>0.99) for all target analytes. The method detection limits of each N-nitrosamines and N-nitrosatable substances released from rubber products were 1.25 and 5.00 mu g/kg, respectively, which are much lower than the requirement of Directive 93/11/EEC (N-nitrosamines: 10 mu g/kg; N-nitrosatable substances: 100 mu g/kg). The average spike recoveries ranged from 80.6% to 95.5% (RSD 4.2%-7.7%, n = 6), from 86.8% to 98.3% (RSD 4.1%-5.8%, n = 6), and from 92.2% to 110.4% (RSD 2.6%-4.3%, n = 6) for the spiked levels of 1.25, 12.5 and 125 mu g/kg, respectively. The total concentrations of seven N-nitrosamines and N-nitrosatable substances in analyzed balloon samples ranged from 0.421 mg/kg to 0.820 mg/kg and from 3.616 mg/kg to 8.437 mg/kg, respectively, which did not comply with the requirement of Directive 93/11/EEC. However, all N-nitrosamines and N-nitrosatable substances were not detected in analyzed teats and soothers samples.
- 出版日期2011-7
- 单位北京大学深圳研究生院; 深圳市计量质量检测研究院
