Using amplified fragment length polymorphism marker to investigate the genetic diversity of local and wild wheat in Syria

作者：Khalil Rami; Li Zhengguo^*

来源：Journal of Food Agriculture and Environment, 2011, 9(2): 345-349.

• Wheat (T durum
• T. aestivum
• T. dicoccoides
• T. urartu)
• landraces
• genetic relationship
• AFLP
• cluster analysis

In this study, the genetic relationship between T durum, T aestivum, T dicoccoides and T urartu was estimated by using Amplified Fragment Length Polymorphism (AFLP) markers. EcoRI and MeI were the selective primers used with one nucleotide extention on the 3' end. The number of unique markers per genotype ranged from 1 to 17, the highest one was revealed by T dicoccoides while the lowest one by T durum. The relationship between the wheat varieties was obtained. The 11 AFLP primer combinations amplified 657 AFLP loci of which 594 (90.4%) were polymorphic. Based on cluster analysis the most distant two cultivars are Senaljamal (Sc) (T durum) and UI (T warm), and the closest two are Bosiedi (Bo) and Aiubieh (Ai).

• 出版日期2011-4
• 单位重庆大学