Application of a simple calorimetric data analysis on the binding study of cyanide ions by Jack bean urease

作者：Behbehani G Rezaei^*; Saboury A A; Mohebbian M; Ghammamy S

来源：Chinese Chemical Letters, 2010, 21(4): 457-460.

DOI：10.1016/j.cclet.2009.10.021

• Isothermal titration calorimetry
• Cyanide ion
• Jack bean urease
• Binding parameter

Cyanide ion was studied as an effector of Jack bean urease (JBU) at 300 K in 30 mmol/L Tris buffer, pH 7 by isothermal titration calorimetry (ITC). The simple novel model was used for CN(-) + JBU interaction over the whole range of CN- concentrations. The binding parameters recovered from the simple novel model were attributed to the cyanide ion interaction. It was found that cyanide ion acted as a noncooperative inhibitor of JBU, and there is a set of 12 identical and independent binding sites for CN(-) ions. The dissociation equilibrium constant is 750 mu mol/L. The molar enthalpy of binding is Delta H = -13.6 kJ mol(-1). The technique used provided an accurate and quick assessment of the effectiveness of the compounds to inhibit Jack bean urease.

• 出版日期2010-4