A low detection limit penicillin biosensor based on single graphene nanosheets preadsorbed with hematein/ionic liquids/penicillinase

作者：Wu, Yueting; Tang, Lele; Huang, Linhong; Han, Zhizhong; Wang, Jian; Pan, Haibo^*

来源：Materials Science & Engineering C-Materials for Biological Applications, 2014, 39: 92-99.

DOI：10.1016/j.msec.2014.02.012

• Penicillin biosensor
• Electrochemistry
• Graphene nanosheets
• Ionic liquids
• Hematein

In this study, we reported on a low detection limit penicillin biosensor with layer-by-layer (LbL) film containing single-graphene nanosheets (SGNs) preadsorbed with hematein, ionic liquids (ILs) and penicillinase. The penicillinase catalyzes the hydrolysis of penicillin to penicilloic acid, where H+ is liberated and monitored amperometrically with hematein as a pH indicator. The SGN-hemateinas/penicillinase biosensor exhibited excellent performance for penicillin in PBS with a wide range from 1.25 x 10(-13) to 7.5 x 10(-3) M, and a low detection limit of 10(-13) M (0.04 ppt, S/N >= 3). Furthermore, the detection of penicillin concentration in real sample (milk) had acceptable accuracy with the assay system.

• 出版日期2014-6-1
• 单位福州大学