科研之友
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摘要
Clinical laboratory tests provide critical information at every stage of the medical decision-making process, and measurement of the activity levels of enzymes such as alkaline phosphatase, lactate dehydrogenase, etc. provide information regarding various body functions such as the liver and gastrointestinal tract. The uncertainty associated with these enzyme measurement processes describes the quality of the measurement process, and therefore methods to improve the quality of the measurement process require minimizing the measurement uncertainty of the enzyme assay. In this study, we develop a mathematical model of the lactate dehydrogenase measurement process, with uncertainty introduced into its parameters that represent the sources of variation in the different components and stages of the measurement process. The Monte Carlo method is then utilized to estimate the uncertainty associated with the model, and therefore the measurement process. An empirical function used to generate estimates of uncertainty for patient samples with unknown activity levels is constructed using the model. The model is then used to quantify the contributions of the individual sources of uncertainty to the net measurement uncertainty and also quantify the effect of uncertainty within the calibration process on the distribution of the measurement result. Copyright (c) 2014 John Wiley & Sons, Ltd. A physics-based mathematical model of measurement uncertainty of the lactate dehydrogenase assay is developed and the Monte Carlo method is used to estimate the measurement uncertainty associated with the model, and therefore the assay. The simulation model is used to quantify the contribution of individual sources of uncertainty to the net measurement uncertainty as well as quantify the effect of uncertainty in the calibration process on the distribution of the measurement result.
- 出版日期2015-1
