Antioxidant, antityrosinase and antibiofilm activities of synthesized peptides derived from Vicia faba protein hydrolysate: A powerful agents in cosmetic application

作者：Karkouch Ines^*; Tabbene Olfa; Gharbi Dorra; Ben Mlouka Mohamed Amine; Elkahoui Salem; Rihouey Christophe; Coquet Laurent; Cosette Pascal; Jouenne Thierry; Limam Ferid

来源：Industrial Crops and Products, 2017, 109: 310-319.

DOI：10.1016/j.indcrop.2017.08.025

• Vicia faba
• Protein hydrolysate
• Peptides
• Antioxidant activity
• Antibiofilm activity
• Tyrosinase inhibitor

The aim of this study was to identify peptides with antioxidant, antityrosinase and antibiofilm activities released from Vicia faba seed proteins hydrolysate. The hydrolysis pattern was performed with trypsin. Firstly, the obtained hydrolysate was fractionated by cation exchange chromatography and the fraction exhibiting the highest biological activity was then analyzed by reverse phase HPLC tandem mass spectrometry. After annotation by Peaks software (BSI, Canada) for de novo peptide sequencing, seven peptides were identified, mainly derived from storage proteins: legumin and vicilin. These peptides were further chemically synthesized to assess their antioxidant capacity, antityrosinase activity and antibiofilm ability against Pseudomonas aeruginosa PA14. Results showed that peptides P5, P6 and P7 identified as LSPGDVLVIPAGYPVAIK, VESEAGLTETWNPNHPELR and EEYDEEKEQGEEEIR respectively, displayed the highest DPPH radical scavenging activity (IC50 = 0.25 L9 mM). P5 was the only peptide able to chelate iron and reduce Fe3+ to Fe2+. Furthermore, peptides P4 (GPLVHPQSQSQSN) and P6 were observed as potent tyrosinase inhibitors with IC50 values of 1 and 0.14 mM respectively. P1, P5, P6 and P7 also showed interesting antibiofilm activity against Pseudomonas aeruginosa PA14 with MBIC50 value ranged from 12 to 35 mu M. Our data suggest that V. faba seed proteins hydrolysate could be potentially used as a source of natural bioactive peptides for cosmetic and pharmaceutical applications.

• 出版日期2017-12-15