Augmentation of Megakaryocyte Expression of Fc gamma RIIa by Interferon gamma

作者：Schneider David J^*; Taatjes Sommer Heidi S

来源：Arteriosclerosis, Thrombosis, and Vascular Biology, 2009, 29(7): 1138-1143.

DOI：10.1161/ATVBAHA.109.187567

• atherosclerosis
• Fc gamma R
• inflammation
• platelets
• megakaryocytes

Objective-The purpose of this study was to identify factors that alter expression of Fc gamma RIIa by megakaryocytes.
Methods and Results-Effects of selected cytokines and growth factors on megakaryocyte expression of Fc gamma RIIa were assessed with phorbol 12-myristate 13-acetate (PMA)-differentiated human erythroleukemia (HEL) cells and with thrombopoietin-differentiated CD34 stem cells and compared with those obtained with myelocytic cell lines and a monocytic cell lines. Expression of Fc gamma RIIa was quantified with the use of Western blots and real-time reverse transcriptase-polymerase chain reaction. Megakaryocyte differentiation was identified by expression of CD41, CD42, and von Willebrand factor with the use of flow cytometry. Interferon (IFN) gamma increased protein expression of Fc gamma RIIa by HEL cells and CD34 stem cells after megakaryocyte differentiation (maximal approximate to 4-fold, P < 0.001 for each). IFN gamma did not increase expression of Fc gamma RIIa by undifferentiated HEL and CD34 cells. Expression of Fc gamma RIIa mRNA was increased (2-fold, P < 0.001) in megakaryocyte-differentiated HEL cells. IFN gamma did not increase expression of Fc gamma RIIa by undifferentiated myelocytic or monocytic cell lines.
Conclusions-IFN gamma appears to selectively increase expression of Fc gamma RIIa by cells exhibiting characteristics of megakaryocytes. This effect of IFN gamma may contribute to greater platelet expression of Fc gamma RIIa in patients with atherosclerotic vascular disease. (Arterioscler Thromb Vasc Biol. 2009; 29: 1138-1143.)

• 出版日期2009-7