Highly sensitive method for the quantification of trans-linolenic acid isomers in trilinolenin of edible oils using an ionic liquid capillary column

作者：Guo, Qin; Jiang, Fan; Jin, Jing; Li, Qingpeng; Wang, Feng^*; Wang, Qiang; Ha, Yiming^*

来源：Journal of the Science of Food and Agriculture, 2017, 97(14): 4697-4703.

DOI：10.1002/jsfa.8337

• trilinolenin
• method
• fatty acid isomers
• gas chromatography
• ionic liquid capillary column

BACKGROUNDThe polarities of linolenic acid isomers are very similar, and only a few studies to date have attempted to separate -linolenic acid (ALA) isomers completely. The aim of this study was to fill this gap by developing and validating an accurate method for the analysis of ALA isomers in trilinolenin at 200, 220 and 240 degrees C using a gas chromatograph-flame ionization detector equipped with an SLB-IL111 capillary column.
RESULTSResults showed that eight ALA isomer standards were separated effectively using these optimized gas chromatographic conditions. The coefficient of determination was r(2) > 0.9994 in the linear range of each ALA isomer. The obtained limits of detection and limits of quantification of the ALA isomers were 0.02-0.08 ppm and 0.05-0.22 ppm, respectively. A high degree of reproducibility and percent recoveries between 96.2% and 106.5%, with coefficients of variation ranging from 0.82% to 0.97%, were achieved.
CONCLUSIONThe developed method has been successfully applied to the analysis of ALA isomers in heated pure trilinolenin as well as to trilinolenin in various edible oils, and the TALA isomerization pathways in heated trilinolenin were verified.

• 出版日期2017-11
• 单位中国农业科学院