A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay

作者：Aloia A L; Duffy L; Pak V; Lee K E; Sanchez Martinez S; Derse D; Heidecker G; Cornetta K; Rein A^*

来源：Gene Therapy, 2013, 20(2): 169-176.

DOI：10.1038/gt.2012.18

• replication-competent gannmaretrovirus
• gene-therapy vectors
• infectivity assay

Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+L- or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S(+)1(-) assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing. Gene Therapy (2013) 20, 169-176; doi:10.1038/gt.2012.18; published online 8 March 2012

• 出版日期2013-2