Marker-assisted development of bread wheat near-isogenic lines carrying various combinations of purple pericarp (Pp) alleles

作者：Gordeeva E I; Shoeva O Y; Khlestkina E K^*

来源：Euphytica, 2015, 203(2): 469-476.

DOI：10.1007/s10681-014-1317-8

• Triticum
• Microsatellites
• Genotyping
• Transcription regulation
• Anthocyanin synthesis

The commercial interest in pigmented wheat grain flows from an understanding that they are nutritionally superior to white kernels. The pigment of purple coloured bread and durum wheat grains results from the accumulation of anthocyanins in the pericarp; its genetic basis is the action of Pp-1 and Pp3 genes. Here, the development of a set of bread wheat near isogenic lines (NILs) carrying various combinations of Pp alleles is described, along with a demonstration of their utility for the genetic dissection of the purple pericarp trait. A marker-assisted backcrossing strategy was based on the use of microsatellite markers linked to Pp3 (chromosome 2A), Pp-A1 (7A) and Pp-D1 (7D). Pp-A1 is a newly uncovered gene of weak effect. A qRT-PCR-based analysis of the anthocyanin synthesis structural genes [Chi (chalcone-flavanone isomerase) and F3h (flavanone 3-hydroxylase)] transcript abundance in the pericarp of the NILs suggested that the Pp genes up-regulate their transcription in contrasting ways. These NILs represent a resource for studying the effect of grain pigmentation on other wheat traits and end products.

• 出版日期2015-5