A new two-phase kinetic model of sporulation of Clonostachys rosea in a new solid-state fermentation reactor

作者：Zhang, Yuan Yuan; Liu, Jun Hong^*; Zhou, Yuan Ming; Zhang, Yu Yan; Liu, Ying; Gong, Ting Yun; Wang, Jing

来源：Process Biochemistry, 2013, 48(8): 1119-1125.

DOI：10.1016/j.procbio.2013.03.009

• Sporulation
• Kinetic model
• Clonostachys rosea
• Solid-state fermentation
• Biological control agent

A new two-phase kinetic model of sporulation of Clonostachys rosea in a new solid-state fermentation (SSF) reactor was proposed. The model including exponential and logistic models was applied to study the simultaneous effect of temperature, initial moisture content, medium thickness and surface porosity of the plastic membrane on C. rosea sporulation. The model fits experimental data very well and allows accurate predictions of spore production. The maximum spore production achieved 3.360 x 10(10) (spores/gDM), about 10 times greater than that in traditional SSF reactor(data not shown). The new reactor can provide two times sporulation surface area. Moisture content can be adjusted by changing the surface porosity to meet the spore production. Two mixings carried out during fermentation makes medium loose and results in a mass of new sporulation surface area. Therefore, the new SSF reactor would have great potential for application in bulk spore production of fungal biocontrol agents.

• 出版日期2013-8
• 单位青岛农业大学; 青岛大学; 青岛科技大学