Adsorption of a porcine reproductive and respiratory syndrome virus DNA vaccine candidate onto biodegradable nanoparticles improves immunogenicity in mice

作者：Du, Luping; Li, Bin; Xu, Xiangwei; Sun, Bing; Pang, Fengjiao; Wen, Libin; Huang, Kehe; He, Kongwang^*

来源：Archives of Virology, 2015, 160(6): 1543-1547.

DOI：10.1007/s00705-015-2396-0

• CATIONIC MICROPARTICLES
• IMMUNE-RESPONSES
• DELIVERY
• GP5
• POLYETHYLENIMINE
• MICROSPHERES
• ENCAPSULATION
• RELEASE
• SYSTEM

Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating viral disease of pigs. Safer and more effective vaccines are urgently needed. In this study, a synthetic ORF5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) was adsorbed onto poly(D, L-lactide-co-glycolide)/polyethylenimine (PLGA/PEI) nanoparticles. We prepared a PLGA-nanoparticle-adsorbed PRRSV DNA vaccine and a PEI-DNA complex. The results showed that these model vaccines could significantly enhance humoral and cellular immune responses when compared with the responses induced by pcDNA3.1-SynORF5, a plasmid construct for expression of PRRSV ORF5. PLGA-branched PEI nanoparticles induced the most efficient immune response. The delivery system and adjuvant provide new models for the development of vaccines against PRRSV.

• 出版日期2015-6
• 单位南京农业大学; 江苏省农业科学院