Monitoring fermentation parameters during phytase production in column-type bioreactor using a new data acquisition system

作者：Spier Michele Rigon; Woiciechowski Adenise Lorenci; Junior Letti Luiz Alberto; Scheidt Gessiel Newton; Sturm Wilerson; Rodriguez Leon Jose Angel; de Carvalho Julio Cesar; Amaral Dergint Dario Eduardo; Soccol Carlos Ricardo^*

来源：Bioprocess and Biosystems Engineering, 2010, 33(9): 1033-1041.

DOI：10.1007/s00449-010-0428-4

• Respirometry
• Phytase
• Citric pulp
• Solid-state fermentation
• Acquisition data system

Fermentation parameters for phytase production in column-type bioreactor were monitored using a new data acquisition system. There are a number of studies reporting phytase production in flasks, but a lack of data about microorganism respiration behaviour during phytase production using column bioreactor. The objectives of this work were the monitoration of fermentation parameters during phytase production and its relation with fungal growth and forced air. Phytase production by A. niger FS3 increased with forced air. The O(2) consumption and CO(2) production during solid-state fermentation were monitored by sensors (in the bottom and top of the columns) linked to controllers, recorded by acquisition software and processed by Fersol2(A (R)) software tool. Phytase synthesis was associated with fungal growth. Therefore, phytase could be used to estimate FS3 biomass formed in citric pulp degradation.

• 出版日期2010-11