A Multi-biological Assay Approach to Assess Microbial Diversity in Arsenic (As) Contaminated Soils

作者：Stazi S R; Moscatelli M C; Papp R; Crognale S; Grego S; Martin M; Marabottini R

来源：Geomicrobiology Journal, 2017, 34(2): 183-192.

DOI：10.1080/01490451.2016.1189015

• Arsenic
• DGGE
• EL-FAME
• MicroResp
• soil enzymes

Microbial genetic, structural and functional diversity was assessed in response to arsenic (As) pollution in a former gold mine soil. Ester-linked fatty acid methyl ester (EL-FAME), quantitative PCR (qPCR), denaturating gradient gel electrophoresis (DGGE), enzyme activities and MicroResp techniques were used.Multivariate analysis showed that As bioavailability in soil was an important driver affecting microbial diversity. Microbial biomass assessed by EL-FAMEs and qPCR generally decreased under higher bioavailable As, as well as enzyme activities and C substrate utilization. Conversely, actinomycetes and fungal biomass increased along with total As content suggesting the selection of more resistant species.

• 出版日期2017