An alternating passage model between mosquitoes and rodents for a genetic study of Japanese encephalitis virus (JEV)

作者：Hou, Wenjun; Li, Chunxiao; Dong, Yande; Xing, Dan; Zhang, Yingmei; Zhang, Hengduan; Shi, Qingming; Guo, Xiaoxia^*; Zhao, Tongyan^*

来源：International Journal of Clinical and Experimental Pathology, 2017, 10(4): 4719-4728.

• Japanese encephalitis virus (JEV)
• SA14
• alternating passage
• experimental model
• genetic mutation

To set up an alternating model between Culex tritaeniorhynchus and Kunming neonatal rats for determining the genomic evolution of Japanese encephalitis virus (JEV). A sequencing analysis of the JEV-SA14 strain was conducted in the rat brain. After 14 cycles of alternate passages, JEV-RNA showed a relatively high mutation rate in the nucleotides over the entire coding genome, and most of the mutations were single synonymous nucleotide substitutions without amino acid changes. Neither insertions nor deletions were observed. In particular, the viral regions involved in viral infectivity and immunogenicity were well conserved. JEV-SA14 has a relatively stable genetic mutation pattern, and the alternating model is useful for laboratory studies on the evolution of JEV, as well as for other arboviruses, such as the Zika virus.

• 出版日期2017