A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study

作者：Yamaguchi Hiroaki; Fujikawa Asuka; Ito Hajime; Tanak Nobuaki; Furugen Ayako; Miyamori Kazuaki; Takahashi Natsuko; Ogura Jiro; Kobayashi Masaki; Yamada Takehiro; Mano Nariyasu; Iseki Ken^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2012, 893: 157-161.

DOI：10.1016/j.jchromb.2012.02.003

• Docetaxel
• LC/ESI-MS/MS
• Rapid and sensitive analysis

Docetaxel is a taxane family antineoplastic agent widely employed in cancer chemotherapy. We developed a liquid chromatography/tandem mass spectrometry method for the determination of docetaxel in human plasma. Plasma samples were deproteinized by acetonitrile containing internal standard paclitaxel. Chromatographic separation was performed on a TSKgel ODS-100V 3 mu m (50 mm x 2.0 mm i.d.) column using a mobile phase composed of acetonitrile-methanol-water-formic acid (50:5:45:0.1, v/v/v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with multi pie reaction monitoring (MRM) mode via electrospray ionization (ESI) source. This method covered a linearity range of 5-5000 ng/mL with the lower limit of quantification of 5 ng/mL. The intra-day precision and inter-day precision (R.S.D.) of analysis were less than 6.7%, and the accuracy (R.E.) was within +/- 9.0% at the concentrations of 5, 20, 200, and 2000 ng/mL. The total run time was 5.0 min. This method was successfully applied for clinical pharmacokinetic investigation.

• 出版日期2012-4-15