A Crucial Role for Hydrogen Sulfide in Oxygen Sensing via Modulating Large Conductance Calcium-Activated Potassium Channels

作者：Li, Qian; Sun, Biying; Wang, Xiaofang; Jin, Zhu; Zhou, Yun; Dong, Li; Jiang, Lin-Hua; Rong, Weifang^*

来源：Antioxidants and Redox Signaling, 2010, 12(10): 1179-1189.

DOI：10.1089/ars.2009.2926

• CAROTID-BODY
• CHEMORECEPTOR CELLS
• ENDOGENOUS PRODUCTION
• KV CHANNELS
• SENSOR
• H2S
• VASOCONSTRICTION
• MECHANISMS
• EXCITATION
• AIRWAY

Hydrogen sulfide (H2S) is an important signaling molecule produced from L-cysteine by cystathionine beta-synthetase (CBS) or cystathionine gamma-lyase (CSE). Here we examined the role of H2S in the oxygen-sensing function of the carotid body chemoreceptors, where the large conductance Ca2+-activated potassium channel (BKCa) plays a key role. In the isolated mouse carotid body/sinus nerve preparations, the H2S donor, NaHS, excited the chemoreceptor afferent nerves in a concentration-dependent manner that was reversed by carbon monoxide donor. The NaHS-evoked excitation was abolished by removing extracellular Ca2+, or using Cd2+, pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid and hexomethonium, suggesting that H2S evokes release of ATP/ACh from type I glomus cells of the carotid body. The chemoreceptor afferent activation by hypoxia was decreased remarkably using CBS inhibitors, amino oxyacetic acid (AOAA) and hydroxylamine, but not CSE inhibitors, propargylglycine and beta-cyano-L-alanine, despite expression of both enzymes in type I glomus cells. In these cells, the BKCa currents were inhibited by hypoxia and such inhibition was mimicked by NaHS and diminished by AOAA. Finally, mice hyperventilated in response to hypoxia, which was prevented by CBS inhibitors. These data suggest that H2S plays a crucial role in mediating the response of carotid body chemoreceptors to hypoxia via modulating the BKCa channels. Antioxid. Redox Signal. 12, 1179-1189.

• 出版日期2010-5
• 单位上海交通大学