Association of-319 C/T and+49 A/G polymorphisms of CTLA-4 gene in patients with hepatitis C virus infection

作者：Enciso Vargas Moises; Ruiz Madrigal Bertha; Francisco Munoz Valle Jose; Yair Morales Balderas Omar; Helena Hernandez Nazara Zamira; Martinez Lopez Erika; Davalos Cobian Carlos; Ramon Padilla Gutierrez Jorge; Rodrigo Torres Baranda Jose; Alfredo Dominguez Rosales Jose; Maldonado Gonzalez Montserrat^*

来源：Medicina Clinica, 2018, 150(7): 251-256.

DOI：10.1016/j.medcli.2017.06.074

• CTLA-4
• Hepatitis C virus
• Single nucleotide polymorphisms

Introduction and objective: Molecular changes in the CTLA-4 gene can modify the ability to control T lymphocyte proliferation, and promote the persistence or elimination of the hepatitis C virus (HCV). We aimed to investigate the frequency and association of -319 C/T and +49 A/G polymorphism in the CTLA-4 gene in patients infected with HCV.
Methods: The CTLA-4 gene polymorphisms (-319 C/T in the promoter region, and +49 A/G in exon 1) were analysed by T-ARMS-PCR in 420 individuals, including 205 chronic HCV infected patients and 215 healthy subjects.
Results: We found a positive association of +49G allele with HCV infection (OR 1.48; 95% CI 1.09-2.02; p=.02), and with males (OR 1.80; 95% CI 1.16-2.79; p=.02), both in chronic disease (without cirrhosis). Also, significant differences in +49 A/G genotypes distribution between HCV infected patients and healthy subjects were shown in a dominant genetic model (GG + GA versus AA; OR 1.57; 95% CI 1.05-2.33; p=.04). No significant differences were observed in the 319 C/T polymorphism between HCV infected patients and healthy subjects. Moreover, -319C/+49G haplotype confers susceptibility to HCV genotype 3 infection (OR 10.68; 95% CI 1.17-96.97; p=.04).
Conclusions: The +49G allele confers susceptibility to HCV infection and with male gender, both in chronic disease. In addition, the -319C/+49G haplotype confers susceptibility to HCV genotype 3 infection. Our results support an important role of the 319 C/T and +49 A/G polymorphisms in HCV infection.

• 出版日期2018-4-13