Development of a multiplex flow-through immunoaffinity chromatography test for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk

作者：Jiang, Wenxiao; Beloglazova, Natalia V.; Wang, Zhanhui; Jiang, Haiyang; Wen, Kai; de Saeger, Sarah; Luo, Pengjie; Wu, Yongning; Shen, Jianzhong^*

来源：Biosensors and Bioelectronics, 2015, 66: 124-128.

DOI：10.1016/j.bios.2014.11.004

• Sulfonamides
• Quinolones
• Immunoassay
• Veterinary drug residue
• Milk
• Food safety

In this paper, a rapid and sensitive multiplex flow-through immunoaffinity chromatography test (FTIACT) was developed for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk. The developed FTIACT method combines the purification, preconcentration and immunochemical detection of multiple antibiotics on the sepharose gel test layers. The use of liposome-encapsulated quantum dots (LQDs) with the FTIACT method exhibited the best results, with limits of detection (LODs) of 1 and 0.5 ng/mL for the sulfonamides (SAs) and quinolones (QNs), respectively, through qualitative analysis (visual detection by the naked eye). In order to achieve low detection limit, the color intensity of the images were converted into relative optical density values to enable a quantitative evaluation. Quantitative analysis of the samples enabled the detection of SAs (0.13 ng/mL) and QNs (0.062 ng/mL) in spiked milk samples. The FTIACT described in this work shows promise as a multiplex immunoassay for the qualitative and quantitative screening of multiple chemical residues in milk.

• 出版日期2015-4-15
• 单位国家食品安全风险评估中心; 深圳大学; 中国农业大学