Water extract of processed Hydrangea macrophylla (Thunb.) Ser. leaf attenuates the expression of pro-inflammatory mediators by suppressing Akt-mediated NF-kappa B activation

作者：Dilshara Matharage Gayani; Jayasooriya Rajapaksha Gendara Prasad Tharanga; Lee Seungheon; Jeong Joon Bum; Seo Yong Taek; Choi Yung Hyun; Jeong Jin Woo; Jang Young Pyo; Jeong Yong Kee; Kim Gi Young^*

来源：Environmental Toxicology and Pharmacology, 2013, 35(2): 311-319.

DOI：10.1016/j.etap.2012.12.012

• Hydrangea macrophylla
• Nitric oxide
• Prostaglandin E-2
• Tumor necrosis factor-alpha
• Nuclear factor-kappa B

Although Hydrangea macrophylla is native to Northeast Asia and widely cultivated in many parts of the world, no studies on its anti-inflammatory effects have been reported. In this study, we evaluated the anti-inflammatory effect of a water extract of processed H. macrophylla leaf (WH) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. WH inhibited the expression of LPS-stimulated pro-inflammatory mediators such as nitric oxide (NO), prostaglandin E-2 (PGE(2)), and tumor necrosis factor-alpha (TNF-alpha), as well as their regulatory genes inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-alpha without any accompanying cytotoxicity. Moreover, WH significantly suppressed the LPS-induced DNA-binding activity of nuclear factor-kappa B (NF-kappa B), as well as the nuclear translocation of the NF-kappa B subunits, p65 and p50 by suppressing of I kappa B alpha phosphorylation and degradation. WH also increased Akt dephosphorylation, leading to the suppression of the DNA-binding activity of NF-kappa B in LPS-stimulated RAW264.7 macrophage cells. Our results indicate that WH downregulates the expression of pro-inflammatory mediators such as NO, PGE(2), and TNF-alpha by suppressing the Akt-mediated NF-kappa B activity in LPS-stimulated RAW264.7 macrophage cells.

• 出版日期2013-3