A rat decellularized small bowel scaffold that preserves villus-crypt architecture for intestinal regeneration

作者：Totonelli Giorgia; Maghsoudlou Panagiotis; Garriboli Massimo; Riegler Johannes; Orlando Giuseppe; Burns Alan J; Sebire Neil J; Smith Virpi V; Fishman Jonathan M; Ghionzoli Marco; Turmaine Mark; Birchall Martin A; Atala Anthony; Soker Shay; Lythgoe Mark F; Seifalian Alexander; Pierro Agostino; Eaton Simon; De Coppi Paolo^*

来源：Biomaterials, 2012, 33(12): 3401-3410.

DOI：10.1016/j.biomaterials.2012.01.012

• Intestinal failure
• Decellularization
• Natural acellular matrix
• Tissue engineering
• Regenerative medicine
• Gut transplantation

Management of intestinal failure remains a clinical challenge and total parenteral nutrition, intestinal elongation and/or transplantation are partial solutions. In this study, using a detergent-enzymatic treatment (DET), we optimize in rats a new protocol that creates a natural intestinal scaffold, as a base for developing functional intestinal tissue. After 1 cycle of DET, histological examination and SEM and TEM analyses showed removal of cellular elements with preservation of the native architecture and connective tissue components. Maintenance of biomechanical, adhesion and angiogenic properties were also demonstrated strengthen the idea that matrices obtained using DET may represent a valid support for intestinal regeneration.

• 出版日期2012-4