A high-sensitivity electrochemical immunosensor based on mobile crystalline material-41-polyvinyl alcohol nanocomposite and colloidal gold nanoparticles

作者：Omidfar Kobra^*; Zarei Hajar; Gholizadeh Fatemeh; Larijani Bagher

来源：Analytical Biochemistry, 2012, 421(2): 649-656.

DOI：10.1016/j.ab.2011.12.022

• Competitive immunosensor
• Polyvinyl alcohol (PVA)
• Mobile crystalline material-41 (MCM-41)
• Gold nanoparticles (AuNPs)
• Human serum albumin (HSA)

A novel competitive immunosensor was developed as a model system using anti-human serum albumin (HSA)-conjugated gold nanoparticles (AuNPs) as an electrochemical label and mobile crystalline material-41 (MCM-41)-polyvinyl alcohol (PVA) mesoporous nanocomposite as an immobilization platform. However, no attempt has yet been made to use the MCM-41 as the supporting electrolyte for the electro-synthesis of nonconducting polymer nanocomposite. This hybrid membrane was evaluated extensively by using field emission scanning electron microscopy (FESEM), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) to determine its physicochemical and electrochemical properties in immunosensor application. FESEM revealed an appropriate and stable attachment between HSA and MCM-41 and also a dense layer deposition of MCM-41-HSA-PVA film onto the electrode surfaces. DPV was developed for quantitative determination of antigen in biological samples. A decrease in DPV responses was observed with increasing concentrations of HSA in standard and real samples. In optimal conditions, this immunosensor based on MCM-41-PVA nanocomposite film could detect HSA in a high linear range (0.5-200 mu g ml(-1)) with a low detection limit of 1 ng ml(-1). The proposed method showed acceptable reproducibility, stability, and reliability and could also be applied to detect the other antigens.

• 出版日期2012-2-15