Phytoceramides and acylated phytosterol glucosides from Pterospermum acerifolium Willd. seed coat and their osteogenic activity

作者：Dixit Preety; Chand Kailash; Khan Mohd Parvez; Siddiqui Jawed Akhtar; Tewari Deepshikha; Ngueguim Florence Tsofack; Chattopadhyay Naibedya; Maurya Rakesh^*

来源：Phytochemistry, 2012, 81: 117-125.

DOI：10.1016/j.phytochem.2012.06.005

• Pterospermum acerifolium
• Acylated phytosterol glucoside
• Phytoceramides
• Osteogenic activity
• Osteoblast

Phytochemical investigation of seed coats of Pterospermum acerifolium afforded two phytoceramides (1, 2) and two acylated phytosterol glucosides (3, 4) together with five known compounds (5-9). Their structures were elucidated on the basis of extensive spectroscopic analysis using 1D, 2D NMR and Mass spectrometry. Compounds 1, 2, 3, and 4 were assessed for their osteogenic activity using primary cultures of osteoblasts harvested from neonatal rat calvaria. Among these compounds, 1 and 2 markedly stimulated osteoblast differentiation assessed by alkaline phosphatase production and osteoblast mineralization by alizarin red-S staining.

• 出版日期2012-9