科研之友
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摘要
A simple and sensitive reversed-phase, high-performance liquid chromatography method using a diode-array detector (DAD) was developed to evaluate the quality of the fruits of Polygonum orientate L., Polygonaceae (Fructus Polygoni Orientalis, FPO) through its chromatographic fingerprint analysis and a simultaneous analysis of its four compounds, namely taxifolin, 5,7,4'-trihydroxy-dihydroflavonol, p-hydroxyphenyl ethanol p-coumarate, and p-hydroxyphenyl ethanol ferulate. The analysis was accomplished on an Agilent Zorbax SB-C-18 column (150 x 4.6 mu m, 5 iim) using gradient elution with acetonitrile and water. The analyses were performed at 25 C with a flow rate of 1.0 mL/min and using ultraviolet (UV) detection at 290 urn. All of the calibration curves showed a strong regression relationship (r(2) 0.9996) that fell within linear ranges, and their recoveries were in the range of 97.67-103.48%. Twenty common peaks were selected as the characteristic peaks in the chromatographic fingerprint analysis to assess the similarities of 49 FPO samples that were obtained from different geographical areas in China. The results indicated that this developed method was accurate, and that it was successfully applied to the analyses of the four compounds and chromatography fingerprints of FPO.
