Embryo pooling: a promising strategy for managing insufficient number of embryos in preimplantation genetic diagnosis

作者：Hu, Xiaokun; Ding, Chenhui; Zhang, Duoduo; Zhou, Wen; Wang, Jing; Zeng, Yanhong; Lv, Jie; Xu, Yan-wen^*; Zhou, Can-Quan^*

来源：Gynecological Endocrinology, 2017, 33(11): 867-871.

DOI：10.1080/09513590.2017.1347778

• Embryo pooling
• preimplantation genetic diagnosis
• vitrification
• cumulative pregnancy rate

This retrospective study evaluated the embryo pooling strategy for managing insufficient number of embryos in preimplantation genetic diagnosis (PGD) through serial vitrification of cleavage-stage embryos from consecutive cycles, and simultaneous blastocysts biopsy in combination with blastocysts obtained in ultimate fresh cycle. A retrospective analysis of the cumulative pregnancy rate of 68 patients underwent cleavage-stage embryos accumulation (Embryo Pooling Group) and 94 patients underwent one stimulation cycle (Control Group) over a 2-year period were conducted. The blastocyst formation rate was comparable between the consecutive cycles and the ultimate cycle in embryo pooling group (56.0 versus 62.0%, p=.078). No significant difference existed between twice-vitrified and once-vitrified warmed blastocysts with respect to implantation rate (50.8 versus 46.3%, p=.658). The implantation rate and cumulative pregnancy rate of embryo pooling group were 49.0 and 67.6%, respectively, which were statistically comparable to the corresponding values of 48.9 and 73.4% obtained in control group. Our study suggests that in patients undergoing ICSI-PGD who do not reach enough embryos in a single stimulation cycle, pooling embryos from consecutive ovarian stimulation cycles is a promising strategy, which can render a cumulative pregnancy rate comparable to those patients who only require one stimulation cycle.

• 出版日期2017
• 单位中山大学