Antioxidant acitivity of low molecular weight hyaluronic acid

作者：Ke, Chunlin; Sun, Lanping; Qiao, Deliang; Wang, Di; Zeng, Xiaoxiong^*

来源：Food and Chemical Toxicology, 2011, 49(10): 2670-2675.

DOI：10.1016/j.fct.2011.07.020

• Antioxidant activity
• Low molecular weight
• Hyaluronic acid
• lmmunosuppressed mice

Two polysaccharides, low molecular weight hyaluronic acid-1 (LMWHA-1) and LMWHA-2, with their molecular weight of 1.45 x 10(5) and 4.52 x 10(4) Da, respectively, were prepared from high molecular weight hyaluronic acid (HA,1.05 x 10(6) Da). LMWHA-1, LMWHA-2 and HA were studied for their antioxidant activities. In vitro antioxidant assay, LMWHA showed strong inhibition of lipid peroxidation and scavenging activities of hydroxyl radical, moderate 1,1-diphenyl-2-picryldydrazyl radical and superoxide anion scavenging activity. In addition, the LMWHA-1 exhibited much stronger antioxidant activity than LMWHA-2 and HA. For antioxidant testing in vivo, LMWHA-1, LMWHA-2 and HA were orally administrated over a period of 7 days in a cyclophosphamide(CY) induced immunosuppressed mice model. As results, administration of LMWHA was able to overcome CY-induced immunosuppression and significantly raised the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC) in immunosuppressed mice. The results showed that the LMWHA, possessing pronounced free radical scavenging and antioxidant activities.

• 出版日期2011-10
• 单位南京农业大学; 蚌埠学院