Proline Rich Polypeptide (PRP-1) Increases the Superoxide-Producing and Ferrihemoglobin Reducing Activities of Cytochrome B-558 Isoforms from Human Lymphosarcoma Tissue Cells

作者：Simonyan G M^*; Galoian K A; Simonyan R M; Simonyan M A; Galoyan A A

来源：Neurochemical Research, 2011, 36(5): 739-745.

DOI：10.1007/s11064-010-0389-7

• Human lymphosarcoma
• Cytochrome b(558)
• Metalloproteins
• Prolin rich polypeptide
• Reactive oxygen species
• NADPH oxidase

The two cytochromes (cyt) b(558) of acidic nature, one-95-100 kDa and another one, 60-70 kDa were isolated for the first time from the human's lymphosarcoma tissue cells using gel filtration and ion exchange chromatography. These hemoproteins possess NADPH dependent O-2 (-)-producing and ferrihemoglobin-reducing activities. The incubation of neuropeptide PRP-1 (5 mu g) with cytochrome b(558), caused elevation of these activities. The gel filtration results indicated possible binding of PRP-1 to these cytochromes b(558). PRP-1 activated both NADPH dependent O-2 (-)-producing and ferriHb-reducing activities of the cyt b(1) (558) and cyt b(2) (558), obtained from human lymphosarcoma tissue cells. One can assume that PRP-1 associated with cyt b(558) on the surface of the tumor cells by increasing both NADPH dependent O-2 (-)-producing and ferriHb-reducing activities of cyt b(558), increases the oxidation- reduction status. Changing the oxidation-reduction status and oxygen homeostasis of the tumor cells by PRP-1 can serve as one of the possible explanation of antitumorigenic effect of this cytokine.

• 出版日期2011-5