Nucleation of platelets with blood-borne pathogens on Kupffer cells precedes other innate immunity and contributes to bacterial clearance

作者：Wong Connie H Y; Jenne Craig N; Petri Bjoern; Chrobok Navina L; Kubes Paul^*

来源：Nature Immunology, 2013, 14(8): 785-+.

DOI：10.1038/ni.2631

Through the use of intravital imaging of the liver, we demonstrate a collaborative role for platelets with Kupffer cells (KCs) in eradicating blood-borne bacterial infection. Under basal conditions, platelets, via the platelet-adhesion receptor GPIb, formed transient %26apos;touch-and-go%26apos; interactions with von Willebrand factor (vWF) constitutively expressed on KCs. Bacteria such as Bacillus cereus and methicillin-resistant Staphylococcus aureus (MRSA) were rapidly caught by KCs and triggered platelets to switch from %26apos;touch-and-go%26apos; adhesion to sustained GPIIb-mediated adhesion on the KC surface to encase the bacterium. Infected GPIb alpha-deficient mice had more endothelial and KC damage than did their wild-type counterparts, which led to more fluid leakage, substantial polycythemia and rapid mortality. Our study identifies a previously unknown surveillance mechanism by which platelets survey macrophages that rapidly converts to a critical host response to blood-borne bacteria.

• 出版日期2013-8