Determination of organophosphorous pesticides in water using in-syringe ultrasound-assisted emulsification and gas chromatography with electron-capture detection

作者：Su Yi Song; Jen Jen Fon^*

来源：Journal of Chromatography A, 2010, 1217(31): 5043-5049.

DOI：10.1016/j.chroma.2010.06.006

• Ultrasound-assisted emulsification microextraction
• Organophosphorus pesticide
• Gas chromatography-microelectron capture detector
• Water sample

An in-syringe ultrasound-assisted emulsification microextraction (USAEME) was developed for the extraction of organophosphorus pesticides (OPPs) from water samples. The OPPs subsequently analyzed gas chromatography (GC) using a microelectron capture detector (mu ECD). Ultrasound radiation was applied to accelerate the emulsification of mu L-level low-density organic solvent in aqueous solutions to enhance the microextraction efficiency of OPPs in the sample preparation for GC-mu ECD. Parameters affecting the efficiency of USAEME, such as the extraction solvent, solvent volume, pH, salt-addition, and extraction time were thoroughly investigated. Based on experimental results, OPPs were extracted from a 5 mL aqueous sample by the addition of 20 mu L toluene as the extraction solvent, followed by ultrasonication for 30s, and then centrifugation for 3 min at 3200 rpm, offered the best extraction efficiency. Detections were linear in the concentration of 0.01-1 mu g/L with detection limits between 1 ng/L and 2 ng/L for OPPs. Enrichment factors ranged from 330 to 699. Three spiked aqueous samples were analyzed, and recovery ranged from 90.1% to 104.7% for farm-field water, and 90.1% to 101.8% for industrial wastewater. The proposed method provides a simple, rapid, sensitive, inexpensive, and eco-friendly process for determining OPPs in water samples.

• 出版日期2010-7-30