Acute hyperhomocysteinaemia blunts endothelial dependent and endothelial independent vasodilatation in diabetic patients

作者：Chousos Ilias; Perrea Despoina; Kyriaki Despoina; Liatis Stavros; Katsilambros Nicholas; Makrilakis Konstantinos^*

来源：Diabetes and Vascular Disease Research, 2010, 7(3): 186-194.

DOI：10.1177/1479164110369687

• diabetes mellitus type 2
• endothelial function
• hyperhomocysteinaemia
• iontophoresis
• laser Doppler flowmetry
• skin microcirculation

The aim of this study was to investigate the effects of acute, methionine-induced hyperhomocysteinaemia on endothelial function of skin microcirculation in diabetic compared with non-diabetic persons. Endothelial-and non-endothelial-dependent vasodilatation of the skin microcirculation were evaluated in 20 persons with type 2 diabetes (59.95 +/- 2.36 years old) and 24 age-and gender-matched controls using laser Doppler flowmetry, during fasting and four hours after 0.1 g/kg methionine-induced acute hyperhomocysteinaemia. Methionine loading caused a comparable increase of homocysteine in the two groups. Four hours after induction of acute hyperhomocysteinaemia, endothelial-dependent vasodilatation (acetylcholine effect) was lower in the diabetic patients compared with the controls ([mean +/- SEM] 8.09 +/- 1.18-fold increase in diabetic patients vs. 11.11 +/- 1.33-fold increase in controls, p=0.027). The same was observed for the endothelial-independent vasodilatation (sodium-nitroprusside effect, 7.55 +/- 0.80-fold increase in the diabetic patients vs. 12.19 +/- 1.26-fold increase in controls, p=0.008). Acute hyperhomocysteinaemia causes a decreased response of both endothelial-dependent and endothelial-independent vasodilatation of the skin microcirculation in diabetic compared with non-diabetic persons. These alterations might contribute to the vascular complications of hyperhomocysteinaemia in diabetes, and especially the microvascular ones.

• 出版日期2010-7