Upregulated expression of cardiac ankyrin repeat protein in human failing hearts due to arrhythmogenic right ventricular cardiomyopathy

作者：Wei, Ying-Jie^*; Cui, Chuan-Jue; Huang, Yin-Xia; Zhang, Xiao-Lling; Zhang, Hao; Hu, Sheng-Shou

来源：European Journal of Heart Failure, 2009, 11(6): 559-566.

DOI：10.1093/eurjhf/hfp049

• Gene microarray
• Cardiac ankyrin repeat protein
• Arrhythmogenic right ventricular cardiomyopathy
• Heart failure
• Marker

Expression of cardiac ankyrin repeat protein (CARP) is augmented in heart failure due to dilated or ischaemic cardiomyopathy. It is unclear whether CARP is upregulated in heart failure due to arrhythmogenic right ventricular cardiomyopathy (ARVC). In the present study, we investigated the expression pattern of CARP and the correlation between CARP and the well-known heart failure marker pro-atrial natriuretic peptide (proANP) in ARVC failing hearts.
Gene microarray analysis demonstrated increased CARP expression in ARVC failing hearts compared with non-failing control hearts, which was further validated by real-time RT-PCR, western blot, and ELISA at the mRNA and protein levels. Fractionation experiments revealed that the upregulation of CARP expression is restricted to the nuclei of residual cardiac cells in ARVC failing hearts. Regression analysis showed a positive correlation between CARP and proANP in ARVC failing hearts.
Augmented CARP expression may be a common molecular event in failing hearts regardless of cardiomyopathic aetiology. The upregulation of nuclear CARP expression and positive correlation between cardiac CARP and proANP suggests that CARP may be used as a genetic marker existing in the nuclei in contrast to proANP existing in the cytosol of cardiac cells in heart failure patients.

• 出版日期2009-6
• 单位中国医学科学院阜外医院; 中国医学科学院北京协和医院