Development of An Electrochemical Impedance Immunosensor for Myoglobin Determination

作者：Sun, Libo; Li, Wenwen; Wang, Maojing; Ding, Wei; Ji, Yang^*

来源：International Journal of Electrochemical Science, 2017, 12(7): 6170-6179.

DOI：10.20964/2017.07.72

• Myoglobin
• Gold nanoparticles
• Impedance immunosensor
• Electrochemical impedance spectroscopy
• Bioelectrode

This work addressed the fabrication of a bioelectrode via the attachment of monolayer-protected Au nanoparticles (AuNPs) onto a 3-aminopropyltriethoxysilane (APTES) self-assembled monolayer (SAM) on an indium-tin-oxide (ITO). An anti-myoglobin antibody (Ab-Mb) was then linked to the AuNPs with a carbodiimide coupling reaction. The terminal bioelectrode was termed as Ab-Mb/AuNPs/APTES/ITO. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were employed for the characterization of the proposed bioelectrode, which displayed an electrochemical impedance response to Ag-Mb in phosphate buffer solution (PBS) with a linear range of 10 ng/mL-1 mu g/mL and an LOD of 2.7 ng/mL.

• 出版日期2017-7
• 单位青岛大学