Assessment of nucleosides as putative tumor biomarkers in prostate cancer screening by CE-UV

作者：Buzatto Adriana Zardini; Silva Mariana de Oliveira; Poppi Ronei Jesus; Colnaghi Simionato Ana Valeria

来源：Analytical and Bioanalytical Chemistry, 2017, 409(13): 3289-3297.

DOI：10.1007/s00216-017-0297-7

• Blood serum
• Micellar electrokinetic capillary chromatography
• Tumor biomarker
• Nucleosides
• Target metabolome
• Prostate cancer
• Carcinogenesis

Cancer is responsible for millions of deaths worldwide, but most base diseases may be cured if detected early. Screening tests may be used to identify early-stage malignant neoplasms. However, the major screening tool for prostate cancer, the prostate-specific antigen test, has unsuitable sensitivity. Since cancer cells may affect the pattern of consumption and excretion of nucleosides, such biomolecules are putative biomarkers that can be used for diagnosis and treatment evaluation. Using a previously validated method for the analysis of nucleosides in blood serum by capillary electrophoresis with UV-vis spectroscopy detection, we investigated 60 samples from healthy individuals and 42 samples from prostate cancer patients. The concentrations of nucleosides in both groups were compared and a multivariate partial least squares-discriminant analysis classification model was optimized for prediction of prostate cancer. The validation of the model with an independent sample set resulted in the correct classification of 82.4% of the samples, with sensitivity of 90.5% and specificity of 76.7%. A significant downregulation of 5-methyluridine and inosine was observed, which can be indicative of the carcinogenic process. Therefore, such analytes are potential candidates for prostate cancer screening.

• 出版日期2017-5