Allogeneic Bone Marrow Transplant from MRL/MpJ Super-Healer Mice Does Not Improve Articular Cartilage Repair in the C57BI/6 Strain

作者：Leonard Catherine A; Lee Woo Yong; Tailor Pankaj; Salo Paul T; Kubes Paul; Krawetz Roman J

来源：PLos One, 2015, 10(6): e0131661.

DOI：10.1371/journal.pone.0131661

Background
Articular cartilage has been the focus of multiple strategies to improve its regenerative/repair capacity. The Murphy Roths Large (MRL/MpJ) "super-healer" mouse demonstrates an unusual enhanced regenerative capacity in many tissues and provides an opportunity to further study endogenous cartilage repair. The objective of this study was to test whether the super-healer phenotype could be transferred from MRL/MpJ to non-healer C57BI/6 mice by allogeneic bone marrow transplant.
Methodology
The healing of 2mm ear punches and full thickness cartilage defects was measured 4 and 8 weeks after injury in control C57BI/6 and MRL/MpJ "super-healer" mice, and in radiation chimeras reconstituted with bone marrow from the other mouse strain. Healing was assessed using ear hole diameter measurement, a 14 point histological scoring scale for the cartilage defect and an adapted version of the Osteoarthritis Research Society International scale for assessment of osteoarthritis in mouse knee joints.
Principal Findings
Normal and chimeric MRL mice showed significantly better healing of articular cartilage and ear wounds along with less severe signs of osteoarthritis after cartilage injury than the control strain. Contrary to our hypothesis, however, bone marrow transplant from MRL mice did not confer improved healing on the C57BI/6 chimeras, either in regards to ear wound healing or cartilage repair.
Conclusion and Significance
The elusive cellular basis for the MRL regenerative phenotype still requires additional study and may possibly be dependent on additional cell types external to the bone marrow.

• 出版日期2015-6-29