An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host

作者：Manderscheid Niko; Bilyk Bohdan; Busche Tobias; Kalinowski Joern; Paululat Thomas; Bechthold Andreas; Petzke Lutz; Luzhetskyy Andriy

来源：Journal of Biotechnology, 2016, 232: 110-117.

DOI：10.1016/j.jbiotec.2016.05.038

• Heterologous expression
• Antibiotic production
• Strain development
• Streptomyces albus J1074
• phi C31-encoded recombination system
• Multiple attachment sites

Streptomyces albus J1074 is a well-known host for heterologous expression of secondary metabolites. To further increase its potential and to study the influence of cluster multiplication, additional phi C31 attachment site was integrated into its genome using a system for transposon mutagenesis. Four secondary metabolite clusters were expressed in strains with different numbers of attachment sites, ranging from one to three copies of the site. Secondary metabolite production was examined and a new compound could be detected, purified and its structure was elucidated.

• 出版日期2016-8-20