NEW HEPATOCELLULAR-CARCINOMA CELL-LINE SUHC-1 ESTABLISHED FROM A PATIENT WITH HEPATITIS-C VIRUS-RNA IN SERUM

作者：YOSHIZAWA K; KIYOSAWA K; USUDA S; YABU K; NAKATSUJI Y; YAMADA S; FURUTA K; TANAKA E; SODEYAMA T; FURUTA S; KANEKO S; NAKANE PK

来源：Japanese Journal of Cancer Research, 1992, 83(8): 871-877.

• HEPATOCELLULAR CARCINOMA CELL LINE
• HEPATITIS-C VIRUS
• HCV-RNA

A new human hepatocellular carcinoma cell (HCC) line, designated SUHC-1, was derived from a Japanese patient with hepatocellular carcinoma having antibody to hepatitis C virus (HCV) and HCV-RNA in his serum, and established in tissue culture. This cell line exhibited typical epithelial cell morphology in culture as observed by phase-contrast and electron microscopy. The SUHC-1 cells produced albumin and alpha-2-macroglobulin. Chromosomal analysis showed several rearrangements at short and long arms of chromosome 1, 17 and 20 (1p-, 1q-, i(1q), i(17q) and 20q+) with a modal number of 91. HCV-RNA was not detected in the supernatant of SUHC-1 cells by nested polymerase chain reaction assay or in the SUHC-1 cells by the in situ hybridization method. We concluded that complete HCV does not exist in the SUHC-1 cell line. The SUHC-1 cell line is the first line of HCC to have been derived from a patient with persistent HCV infection, and may provide a suitable model for studies of hepatocarcinogenesis related to HCV.

• 出版日期1992-8