An ultrasensitive electrochemiluminescence sensor based on reduced graphene oxide-copper sulfide composite coupled with capillary electrophoresis for determination of amlodipine besylate in mice plasma

作者：Wei, Yanfen; Wang, Hao; Sun, Shuangjiao; Tang, Lifu; Cao, Yupin; Deng, Biyang^*

来源：Biosensors and Bioelectronics, 2016, 86: 714-719.

DOI：10.1016/j.bios.2016.07.068

• Graphene
• Copper sulfide
• Amlodipine besylate
• Capillary electrophoresis
• Electrochemiluminescence
• Mice plasma

A new electrochemiluminescence (ECL) sensor based on reduced graphene oxide-copper sulfide (rGO-CuS) composite coupled with capillary electrophoresis (CE) was constructed for the ultrasensitive detection of amlodipine besylate (AML) for the first time. In this work, rGO-CuS composite was synthesized by one-pot hydrothermal method and used for electrode modification. The electrochemical and ECL behaviors of the sensor were investigated. More than 5-fold enhance in ECL intensity was observed after modified with rGO-CuS composite. The results can be ascribed to the presence of rGO-CuS composite on the electrode surface that facilitates the electron transfer rate between the electroactive center of Ru (bpy)(3)(2+) and the electrode. The ECL sensor was coupled with CE to improve the selectivity and the CE-ECL parameters that affect separation and detection were optimized. Under the optimum conditions, the linear ranges for AML was 0.008-5.0 mu g/mL with a detection limit of 2.8 ng/mL (S/N=3). The method displayed the advantages of high sensitivity, good selectivity, wide linear range, low detection limit and fine reproducibility, and was used to analyze AML in mice plasma with a satisfactory result, which holds a great potential in the field of pharmaceutical analysis.

• 出版日期2016-12-15
• 单位广西师范大学