科研之友
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摘要
Gastrodia rhizome is a famous Chinese medicinal plant and has been widely used in China for centuries. In this paper, we have, preparatively seperated gastrodin from the rats' urine after metabolism. Male Wistar rats were orally given crude gastrodin water solution and their urine was collected for continuously 6 h. The collected urine was absorbed with Macroporous resin D101 and desorbed with ethanol water solution (5:95, v/v) to give crude gastrodin. The crude gastrodin was then purified by silica gel column chromatography to get 262 mg gastrodin over 98 % in purity. The total recovery yield of gastrodin was ca. 70.4 %. Though low recovery, our results provided a potential way to recycle gastrodin because of huge amounts need of Gastrodia rhizome in China every year.
