Aminoethylated chitooligomers and their apoptotic activity on AGS human cancer cells

作者：Karagozlu Mustafa Zafer; Karadeniz Fatih; Kong Chang Suk; Kim Se Kwon^*

来源：Carbohydrate Polymers, 2012, 87(2): 1383-1389.

DOI：10.1016/j.carbpol.2011.09.034

• Aminoethyl chitooligomers
• Apoptosis
• AGS

In this study, the ability of aminoethylation of chitooligomers (COS) to inhibit the proliferation of AGS human gastric adenocarcinoma cells was evaluated using COS with lower molecular weight (%26lt;1 kDa). As water-soluble aminoderivatized COS derivatives, aminoethyl-COS (AE-COS), dimethyl aminoethyl-COS (DMAE-COS) and diethyl aminoethyl-COS (DEAE-COS) were synthesized and confirmed by their IR spectra results in comparison to previous study. Aminoderivatized COS-induced cell death was characterized by cell viability, changes in nuclear morphology and cell morphology. All aminoderivatized COS significantly inhibited cell proliferation of AGS cancer cells in a dose-dependent manner. Moreover, protein and gene expression levels of the regulators involved in apoptosis pathway such as Caspase-9, Bax, p53 and p21 were examined using RT-PCR and Western blot analysis. The exposure of synthesized COS derivatives to AGS cells induced apoptotic activity. Therefore, the present results suggest that all aminoderivatized COS derivatives have a promising potential as valuable as cancer chemopreventive agents.

• 出版日期2012-1-15