Determination of Tetracycline Antibiotic Residues in Honey and Milk by Miniaturized Solid Phase Extraction Using Chitosan-Modified Graphitized Multiwalled Carbon Nanotubes

作者：Xu, Jing-Jing; An, Mingrui; Yang, Rui; Tan, Zhijing; Hao, Jie; Cao, Jun^*; Peng, Li-Qing; Cao, Wan

来源：Journal of Agricultural and Food Chemistry, 2016, 64(12): 2647-2654.

DOI：10.1021/acs.jafc.6b00748

• chitosan
• graphitized multiwalled carbon nanotubes
• miniaturized solid phase extraction
• tetracycline antibiotics
• ultrahigh-performance liquid chromatography
• quadrupole time-of-flight mass spectrometry

A rapid, simple, and strongly selective miniaturized solid phase extraction (SPE) technique, requiring only small amounts of sorbent (24 mg) and elution solvent (600 mu L), coupled with ultrahigh-performance liquid chromatography and quadrupole time-of-flight mass spectrometry was developed for detecting tetracycline antibiotics. These analytes were extracted from honey and milk using chitosan-modified graphitized multiwalled carbon nanotubes (G-MWNTs) as the solid sorbent and acetonitrile/acetic acid (8:2, v/v) as the eluent in miniaturized SPE. Under the optimum experimental conditions, a satisfactory linearity (r(2) > 0.992) was obtained, and the limits of detection were in the range of 0.61-10.34 mu g/kg for the analytes. The mean recoveries of the five tetracycline antibiotic residues in the real samples were between 81.5 and 101.4%. The results demonstrated that chitosan-modified G-MWNTs comprise a promising material for the enrichment of tetracycline antibiotics from complex food matrices.

• 出版日期2016-3-30
• 单位杭州师范大学