A discontinuous autoinhibitory module masks the A1 domain of von Willebrand factor

作者：Deng W; Wang Y; Druzak S A; Healey J F; Syed A K; Lollar P; Li R^*

来源：Journal of Thrombosis and Haemostasis, 2017, 15(9): 1867-1877.

DOI：10.1111/jth.13775

• blood platelet
• ristocetin
• tandem mass spectrometry
• Type 2B von Willebrand disease
• von Willebrand factor

Background How von Willebrand factor (VWF) senses and responds to shear flow remains unclear. In the absence of shear flow, VWF or its fragments can be induced to bind spontaneously to platelet GPIb.
Objectives To elucidate the auto-inhibition mechanism of VWF.
Methods Hydrogen-deuterium exchange (HDX) of two recombinant VWF fragments expressed from baby hamster kidney cells were measured and compared.
Results The shortA1 protein contains VWF residues 1261-1472 and binds GPIb with a significantly higher affinity than the longA1 protein that contains VWF residues 1238-1472. Both proteins contain the VWF A1 domain (residues 1272-1458). Many residues in longA1, particularly those in the N- and C-terminal sequences flanking the A1 domain, and in helix 1, loops 12 and 32, demonstrated markedly reduced HDX compared with their counterparts in shortA1. The HDX-protected region in longA1 overlaps with the GPIb-binding interface and is clustered with type 2B von Willebrand disease (VWD) mutations. Additional comparison with the HDX of denatured longA1 and ristocetin-bound longA1 indicates the N- and C-terminal sequences flanking the A1 domain form cooperatively an integrated autoinhibitory module (AIM) that interacts with the HDX-protected region. Binding of ristocetin to the C-terminal part of the AIM desorbs the AIM from A1 and enables longA1 binding to GPIb.
Conclusion The discontinuous AIM binds the A1 domain and prevents it from binding to GPIb, which has significant implications for the pathogenesis of type 2B VWD and the shear-induced activation of VWF activity.

• 出版日期2017-9