Degradation of methomyl by the combination of Aminobacter sp MDW-2 and Afipia sp MDW-3

作者：Zhang, C.; Yang, Z.; Jin, W.; Wang, X.; Zhang, Y.; Zhu, S.; Yu, X.; Hu, G.; Hong, Q.^*

来源：Letters in Applied Microbiology, 2017, 64(4): 289-296.

DOI：10.1111/lam.12715

• Afipia sp MDW-3
• Aminobacter sp MDW-2
• combination
• degradation pathway
• methomyl

Methomyl (S-methyl N-(methylcarbamoyloxy) thioacetimidate) is a kind of oxime carbamate insecticide. It is considered to be extremely toxic to nontarget organism. To date, no pure culture or consortium has been reported to have the ability to degrade methomyl completely. In this study, a methomyl-degrading enrichment E1 was obtained by using the sludge from the wastewater-treating system of a pesticide manufacturer as the original inoculant. Two bacterial strains named MDW-2 and MDW-3 were isolated from this enrichment, and they were preliminarily identified as Aminobacter sp. and Afipia sp. respectively. Strains MDW-2 and MDW-3 could coexist and degrade 50 mg l(-1) methomyl completely within 3 days by the cooperative metabolism. Methomyl was first converted to methomyl oxime and methylcarbamic acid by strain MDW-2, and the latter could be used as the carbon source for the growth of strain MDW-2. But methomyl oxime could not be sequentially degraded by strain MDW-2. However, it could be degraded and used as the carbon source by strain MDW-3.

• 出版日期2017-4
• 单位南京农业大学