科研之友
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摘要
In acetic acid buffer solution, glucose oxidase (GOD) catalyzed the dissolved oxygen oxidation of glucose to form H2O2, In succession, horseradish peroxidase (HRP) catalyzed the H2O2 oxidizing excess I- to form I-3(-). The I-3(-) combined with a cationic surfactant (CS) Such as tetradecyl dimethyl benzyl ammonium chloride (TDMBA) to produce TDMBA-I-3 association particles that exhibited the strongest resonance scattering (RS) peak at 460 nm. The enhanced RS intensity at 460 nm was linear with glucose concentration in the range of 2.0 x 10(-8) - 2.0 x 10(-6) mol/L, with a detection limit of 8.5 x 10(-9) mol/L. The glucose in serum samples were assayed by the enzyme catalytic RS assay and by spectrophotometry. The results of both assays showed a close correlation. This assay has simplicity, sensitivity and good specificity for quantitative determination of glucose.
