Applications of Hadamard transform-gas chromatography/mass spectrometry to the detection of acetone in healthy human and diabetes mellitus patient breath

作者：Fan Gang Ting; Yang Chien Lin; Lin Cheng Huang^*; Chen Chien Chung; Shih Chung Hung

来源：Talanta, 2014, 120: 386-390.

DOI：10.1016/j.talanta.2013.12.025

• Acetone
• Human breath
• Diabetes mellitus (DM)
• Hadamard transform
• Gas chromatography/mass spectrometry
• Glycohemoglobin A1c (HbA1c)

The Hadamard transform-gas chromatography/mass spectrometry (HT-GC/MS) technique was successfully employed to detect acetone, a biomarker for diabetes mellitus (DM) prediction, in human breath. Samples of exhaled breath were collected from four DM patients (one type-I and three type-II) and eight volunteers (nondiabetic healthy subjects), respectively. The gas samples, without any pretreatment, were simultaneously injected into a GC column through a Hadamard-injector based on Hadamard codes. Under optimized conditions, when cyclic S-matrix orders of 255, 1023 and 2047 were used, the SIN ratios of the acetone signals were substantially improved by 8.0-, 16.0- and 22.6-fold, respectively; these improvements are in good agreement with theoretically calculated values. We found that the breath acetone concentration levels in the four DM patients and the eight volunteers ranged from 1 to 10 ppmv and 0.1 to 1 ppmv, respectively.

• 出版日期2014-3